Details

Autor(en) / Beteiligte

• Catt, J.W.
• Vithanage, H.I.M.V.
• Callow, J.A.
• Callow, M.E.
• Evans, L.V.

Titel

Fertilization in brown algae: V. Further investigations of lectins as surface probes

Ist Teil von

• Experimental cell research, 1983-08, Vol.147 (1), p.127-133

Ort / Verlag

Orlando, FL: Elsevier Inc

Erscheinungsjahr

1983

Link zum Volltext

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• The binding of FITC- (fluorescein isothiocyanate), Au- and 125I-labelled lectins (conA, RCA 120 ( Ricinus communis agglutinin, MW 120000) and FBP (fucose-binding protein from Lotus tetragonobolus)) to gametes of Fucus serratus and their physiological effects on fertilization have been studied. Results indicate that eggs strongly bind FITC- and Aulabelled conA and RCA 120, whilst FITC-FBP binds strongly to sperm. All three iodinated lectins bound to eggs but this was apparently non-specific and similar in magnitude to the binding of iodinated bovine serum albumin. The results suggested the possibility of two distinct types of lectin receptor on egg surfaces: non-specific, highly abundant receptors and less abundant, specific receptors, possibly locally aggregated. All three lectins inhibit fertilization, FBP being the most effective.