Details

Autor(en) / Beteiligte

• Porchet, N.
• Nguyen Van Cong
• Dufosse, J.
• Audie, J.P.
• Guyonnet-Duperat, V.
• Gross, M.S.
• Denis, C.
• Degand, P.
• Bernheim, A.
• Aubert, J.P.

Titel

Molecular cloning and chromosomal localization of a novel human tracheo-bronchial mucin cDNA containing tandemly repeated sequences of 48 base pairs

Ist Teil von

• Biochemical and biophysical research communications, 1991-03, Vol.175 (2), p.414-422

Ort / Verlag

San Diego, CA: Elsevier Inc

Erscheinungsjahr

1991

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• A λgt11 cDNA library constructed from human tracheo-bronchial mucosa was screened with a polyclonal antiserum raised to chemically deglycosylated pronase glycopeptides from human bronchial mucins. Out of 20 positives clones, one partial cDNA clone was isolated and allowed to map a novel human tracheo-bronchial mucin gene. It contains 48 nucleotide tandem repeats quite perfectly identical which encodes a protein containing about 50% of hydroxy amino-acids. This clone hybridized to polydisperse messages produced by human tracheo-bronchial and human colonic mucosae. The gene (proposed name MUC 4) from which cDNA is derived maps to chromosome 3.