Details

Autor(en) / Beteiligte

• GANDY, S. E
• GREBB, J. A
• POSNER, J. B
• GREENGARD, P
• ROSEN, N
• ALBERT, K. A
• DEVINSKY, O
• BLUMBERG, H
• ANDERSON, N
• CEDARBAUM, J. M
• POTTER, R. J
• SEDVALL, G

Titel

General assay for phosphoproteins in cerebrospinal fluid : a candidate marker for paraneoplastic cerebellar degeneration

Ist Teil von

• Annals of neurology, 1990-12, Vol.28 (6), p.829-833

Ort / Verlag

Hoboken: Willey-Liss

Erscheinungsjahr

1990

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• The components of protein phosphorylation systems (protein kinases, protein phosphatases, and their phosphoprotein substrates) are highly enriched in neuronal cells compared with other cell types. We exploited this relative neuronal enrichment of protein phosphorylation system components to develop a general assay technique for putative protein kinase substrates (phosphoproteins) in human cerebrospinal fluid. Using this cerebrospinal fluid phosphoprotein assay, we have detected a putative protein kinase C substrate protein of apparent Mr 60 kd in 6 of 14 patients with paraneoplastic cerebellar degeneration but not in any of 55 patients with a variety of other neurological diseases. Phosphoproteins in cerebrospinal fluid may provide novel and unique markers for the diagnosis or staging of neuronal diseases as well as offer potential insights into the biochemical characterization of affected neuronal populations.