Details

Autor(en) / Beteiligte

• Phillips, J.E.
• Chaffee, R.R.J.

Titel

Restorative effects of spermine on oxidative phosphorylation and respiration in heat-aged mitochondria

Ist Teil von

• Biochemical and biophysical research communications, 1982-09, Vol.108 (1), p.174-181

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

1982

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• Samples of fresh rat liver mitochondria were heat-aged in 37°C isotonic sucrose sufficiently to cause approximately a 30% reduction in the ADP:O ratio when using beta-hydroxybutyric acid as substrate. Subsequent polarographic assays showed spermine concentrations between 0.0157 and 0.250 mM to cause a striking linear restoration of the ADP:O ratio. Spermine also improves both the respiratory rate during the conversion of ADP to ATP and the respiratory control ratio of heat-aged mitochondria. The effects of spermine on the respiratory rate after ADP to ATP conversion vary depending on the spermine concentration. Spermine has no significant effects on mitochondrial respiration prior to addition of ADP.