Details

Autor(en) / Beteiligte

• Ōura, Chikayoshi
• Toshimori, Kiyotaka

Titel

Ultrastructural Studies on the Fertilization of Mammalian Gametes

Ist Teil von

• International Review of Cytology, 1990, Vol.122, p.105-151

Ort / Verlag

United States: Elsevier Science & Technology

Erscheinungsjahr

1990

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• This chapter summarizes the current knowledge of the ultrastructural aspects of gamete interactions. The events associated with fertilization are subjected to ultrastructural analysis by transmission electron microscopy (TEM) and scanning electron microscopy (SEM) as phenomena that involve two single, highly specialized cells (the sperm and egg), and are of fundamental importance for the production of new individuals and the conservation of the species. The fertilization events are analyzed by reproductive biologists, improved existing methods, and developed new sophisticated techniques. These include in vitro fertilization (IVF) procedures and various cytochemical techniques using specific membrane probes, such as lectins, antibiotic filipin, and monospecific antisera. The chapter provides information to understand the relationship between the bioactive molecules and the cell structures. The significance and clinical application of IVF techniques such as zona-drilledova, in vivo information is required to compare such biological events as the fine structure of the gametes between in vivo and in vitro conditions, and between human and other species, to assess the mechanism.