Details

Autor(en) / Beteiligte

• Pelletier, Marc P.
• Giaid, Adel
• Sivaraman, Sujith
• Dorfman, Julia
• Li, Carlos M.
• Philip, Anie
• Chiu, Ray C.-J.

Titel

Angiogenesis and growth factor expression in a model of transmyocardial revascularization

Ist Teil von

• The Annals of thoracic surgery, 1998-07, Vol.66 (1), p.12-18

Ort / Verlag

Netherlands: Elsevier Inc

Erscheinungsjahr

1998

Quelle

Electronic Journals Library

Beschreibungen/Notizen

• Background. The mechanism by which transmyocardial revascularization (TMR) exerts a beneficial effect remains unknown. We hypothesize that the myocardial punctures of TMR cause a myocardial injury, leading to an angiogenic response mediated by a number of growth factors. Methods. Fifty-three rats underwent ligation of the left coronary artery. Group I (n = 25) served as controls, whereas group II (n = 28) underwent concomitant TMR by the creation of six transmural channels with a 25-gauge needle in the ischemic zone. Surviving animals in both groups were sacrificed at intervals of 1, 2, 4, and 8 weeks (n = 5 in each subgroup). Immunohistochemistry in the infarct areas was performed for factor VIII to assess vascular density. Immunohistochemistry using specific antibodies was also performed for transforming growth factor-β, basic-fibroblast growth factor, and vasoendothelial growth factor. Growth factor expression was quantitated by comparing areas of staining (in mm 2) with computerized morphometric analysis. Results. Mortality was similar in both groups (5/25 versus 8/28; not significant). Group II had significantly greater vascular density than group I (5.65 versus 4.06 vessels/high-power field; p < 0.001), with a peak at 1 week postoperatively (9.12 versus 5.56 vessels/high-power field; p < 0.0001) in both groups. Overall, levels of both transforming growth factor-β and basic-fibroblast growth factor were significantly higher in the TMR group compared with the control group (0.207 versus 0.141 mm 2/mm 2, p < 0.05; and 0.125 versus 0.099 mm 2/mm 2, p < 0.05). Conclusions. This model of TMR is associated with a significant angiogenic response, which appears to be mediated by the release of certain angiogenic growth factors such as transforming growth factor-β and basic-fibroblast growth factor. With the long-term patency of laser-created myocardial channels in clinical TMR increasingly in doubt, its mechanism of myocardial revascularization may be similar to that observed in our model.