Nature biotechnology, 1998-07, Vol.16 (7), p.642-646
1998
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Details
- Autor(en) / Beteiligte
- Titel
- Transgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells
- Ist Teil von
- Nature biotechnology, 1998-07, Vol.16 (7), p.642-646
- Ort / Verlag
- New York, NY: Nature
- Erscheinungsjahr
- 1998
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We have developed a method, using nuclear transplantation, to produce transgenic embryonic stem (ES)-like cells from fetal bovine fibroblasts. These cells, when reintroduced into preimplantation embryos, differentiated into derivatives from the three embryonic germ layers, ectoderm, mesoderm, and endoderm, in 5-month-old animals. Six out of seven (86%) calves born were found to be chimeric for at least one tissue. These experiments demonstrate that somatic cells can be genetically modified and then de-differentiated by nuclear transfer into ES-like cells, opening the possibility of using them in differentiation studies and human cell therapy.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1087-0156eISSN: 1546-1696DOI: 10.1038/nbt0798-642Titel-ID: cdi_proquest_miscellaneous_79995088
- Format
- –
- Schlagworte
- ANIMAL TRANSGENIQUE, ANIMALES TRANSGENICOS, Animals, Animals, Genetically Modified, beta-Galactosidase - analysis, Biological and medical sciences, Biotechnology, Blastocyst - physiology, BOVIN, CATTLE, Cattle - genetics, Cell Differentiation, Cell Fusion, CHIMAERAS, Chimera, CHIMERE, DESARROLLO EMBRIONARIO, DEVELOPPEMENT EMBRYONNAIRE, Ectoderm - physiology, Embryo Transfer, EMBRYONIC DEVELOPMENT, EMBRYONIC STEM CELLS, Endoderm - physiology, Fundamental and applied biological sciences. Psychology, GANADO BOVINO, Gene Transfer Techniques, Genetic engineering, Genetic technics, Genetic Therapy, Mesoderm - physiology, Methods. Procedures. Technologies, NOYAU CELLULAIRE, NUCLEI, NUCLEO, NUCLEUS, QUIMERA, Stem Cells - physiology, TRANSGENIC ANIMALS, Transgenic animals and transgenic plants, TRANSPLANTATION, TRASPLANTES