Details

Autor(en) / Beteiligte

• Serna, Sonia
• Etxebarria, Juan
• Ruiz, Nerea
• Martin-Lomas, Manuel
• Reichardt, Niels-Christian

Titel

Construction of N-Glycan Microarrays by Using Modular Synthesis and On-Chip Nanoscale Enzymatic Glycosylation

Ist Teil von

• Chemistry : a European journal, 2010-11, Vol.16 (44), p.13163-13175

Ort / Verlag

Weinheim: WILEY-VCH Verlag

Erscheinungsjahr

2010

Quelle

MEDLINE

Beschreibungen/Notizen

• An effective chemoenzymatic strategy is reported that has allowed the construction, for the first time, of a focused microarray of synthetic N‐glycans. Based on modular approaches, a variety of N‐glycan core structures have been chemically synthesized and covalently immobilized on a glass surface. The printed structures were then enzymatically diversified by the action of three different glycosyltransferases in nanodroplets placed on top of individual spots of the microarray by a printing robot. Conversion was followed by lectin binding specific for the terminal sugars. This enzymatic extension of surface‐bound ligands in nanodroplets reduces the amount of precious glycosyltransferases needed by seven orders of magnitude relative to reactions carried out in the solution phase. Moreover, only those ligands that have been shown to be substrates to a specific glycosyltransferase can be individually chosen for elongation on the array. The methodology described here, combining focused modular synthesis and nanoscale on‐chip enzymatic elongation, could open the way for the much needed rapid construction of large synthetic glycan arrays. Go nano! Arrays of immobilised carbohydrates can be enzymatically processed with the help of glycosyl transferases in nanodroplets placed on top of individual spots. This methodology opens the way for the stereospecific on‐chip construction of glycan arrays with very high ligand densities by using immobilised synthetic glycan scaffolds and minute amounts of recombinant glycosyltransferases (see figure).