Details

Autor(en) / Beteiligte

• CARDABA, B
• DEL POZO, V
• PALOMINO, P
• LAHOZ, C
• JURADO, A
• GALLARDO, S
• CORTEGANO, I
• ARRIETA, I
• DEL AMO, A
• TRAMON, P
• FLORIDO, F
• SASTRE, J

Titel

Olive pollen allergy: searching for immunodominant T-cell epitopes on the Ole e 1 molecule

Ist Teil von

• Clinical and experimental allergy, 1998-04, Vol.28 (4), p.413-422

Ort / Verlag

Oxford BSL: Blackwell Science Ltd

Erscheinungsjahr

1998

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Background The amino‐acid and nucleotide sequence of Ole e 1 (the major antigen of olive pollen) has been described and the IgE antibody response to this major allergen was associated with DR7/DQ2 antigens. With this previous data we try to define the T‐cell epitopes implicated in Ole e 1 reactivity. Objectives To study the recognition of T cells (derived from allergic and non‐allergic Ole e 1 patients) to Ole e 1 synthetic peptides in order to define immunodominant T‐cell epitopes. Methods We have compared the proliferative response of the peripheral blood mononuclear cells from Ole e 1 sensitized patients vs. non‐sensitized controls, induced by 14 Ole e 1 synthetic peptides. Thirty subjects were classified in two groups: group 1 (non‐responders against Ole e 1, n = 16) and group 2 (Ole e 1 responders, n = 14), according to their clinical parameters and the presence or not in their sera of the significant Ole e 1 IgE antibody levels. Results Our results shown that it is possible to find T cells reactive to Ole e 1 peptides in patients with and without significant levels of Ole e 1 IgE antibodies. However, the percentage of response was higher in patients with IgE antibodies 71.4% vs 25%), and the recognition profile was different: the control group showed a broad reactivity pattern, in contrast, the response by the ‘Ole e 1 responders’ group was mainly directed against three peptides of the carboxi‐terminal region, peptides 10 (91–102), 12 (109–120) and 13 (119–130), with a response frequency of 35.7, 28.5 and 28.5%, respectively. By direct and inhibition test no antibody response was found against the synthetic peptides. Conclusions Our data suggest that the regions between 91 and 102 and 109–130 amino‐acids on the Ole e 1 molecule are immunodominant T‐cell epitopes. These epitopes are not recognized by IgE antibodies.