Details

Autor(en) / Beteiligte

• Ronchetti, I.Pasquali
• Alessandrini, A.
• Baccarani Contri, M.
• Fornieri, C.
• Mori, G.
• Quaglino, D.
• Valdrè, U.

Titel

Study of elastic fiber organization by scanning force microscopy

Ist Teil von

• Matrix biology, 1998-04, Vol.17 (1), p.75-83

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

1998

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• Elastic fibers of beef ligamentum nuchae were observed by atomic force microscopy and data compared with those obtained by conventional and freeze-fracture electron microscopy. Fresh isolated elastin fibers as well as thin sections of ligament fragments, which were fixed and embedded either in relaxed or in stretched conditions, were analysed. The results confirm that, at least in beef ligamentum nuchae, elastic fibers consist of beaded filaments which can be oriented by stretching in the direction of the force applied. Moreover, atomic force microscopy revealed that these beaded filaments are laterally connected by periodical bridges which become more pronounced upon stretching. The data clearly show that elastin molecules are organized in a rather ordered array, at least at the super-molecular level, and a depiction of the elastin organization in beef ligamentum nuchae is attempted.