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Differential expression pattern of retinoid X receptors in adult murine testicular cells implies varying roles for these receptors in spermatogenesis
Ist Teil von
Biology of reproduction, 1998-06, Vol.58 (6), p.1351-1356
Ort / Verlag
Madison, WI: Society for the Study of Reproduction
Erscheinungsjahr
1998
Quelle
MEDLINE
Beschreibungen/Notizen
Retinoids have previously been shown to be crucial for normal spermatogenesis. The role of retinoic acid receptors has been
studied, but relatively little is known about the function of retinoid X receptors (RXRs). To gain more insight in the function
of RXRs during spermatogenesis, the cellular localization of RXRs in the mouse testis was examined using immunohistochemistry
and RNase protection assays. In both normal and vitamin A-deficient (VAD) testes, a strong immune response to an RXRalpha
antibody occurred in Leydig cells, peritubular myoid cells, and A spermatogonia. Weaker signals were found in spermatocytes
and spermatids. In normal testes, an RXRbeta antibody gave a reaction in Leydig cells, and, to a lesser extent, in Sertoli
cells, A spermatogonia, pachytene spermatocytes, and spermatids. In Leydig cells, a cytoplasmatic signal was found in addition
to the nuclear signal. In the VAD testis, only Leydig cells and A spermatogonia were positive, which indicates that RXRbeta
expression may be dependent on the retinoid status. Previous studies have shown RXRgamma mRNA expression in the mouse testis
at a low level. Nevertheless, an RXRgamma antibody caused a strong immune response in interstitial cells and in A spermatogonia,
and a weak signal in pachytene spermatocytes. These immunohistochemical data were supported by the results of RNase protection
assays on mRNA of testicular cell isolations. In conclusion, the different RXRs in the mouse testis have distinct expression
patterns, suggesting that they may have different functions.