Details

Autor(en) / Beteiligte

• Neelissen, J A
• Arth, C
• Schrijvers, A H
• Junginger, H E
• Boddé, H E

Titel

Validation of freeze-drying to visualize percutaneous 3H-estradiol transport: the influence of skin hydration on the efficacy of the method

Ist Teil von

• Skin pharmacology and applied skin physiology, 1998-01, Vol.11 (1), p.11-22

Ort / Verlag

Switzerland

Erscheinungsjahr

1998

Quelle

MEDLINE

Beschreibungen/Notizen

• A study was made of the validity of freeze-drying to visualize the distribution of 3H-estradiol in human stratum corneum after topical application of a dry dose, a patch or a buffer solution. Each of these donor formulations was applied to human dermatomed skin for 24 h using Franz permeation cells. Subsequently, small pieces of skin were subjected to cryofixation, freeze-drying, osmium tetroxide vapor fixation, Spurr resin embedding and electron microscopic autoradiography. Stratum corneum from dry dose and patch application experiments was well preserved by freeze-drying, allowing an accurate localization of 3H-estradiol. In contrast, stratum corneum from buffer solution experiments suffered from cryofixation artifacts due to excessive hydration of the skin. The corresponding autoradiographs showed strong redistribution of 3H-estradiol. Thus, the visualization method under investigation has its limitations regarding the hydration level of the skin.