Details

Autor(en) / Beteiligte

• FRANK, S. J
• NIKLINSKA, B. B
• ORLOFF, D. G
• MERCEP, M
• ASHWELL, J. D
• KLAUSNER, R. D

Titel

Structural mutations of the T cell receptor ζ chain and its role in T cell activation

Ist Teil von

• Science (American Association for the Advancement of Science), 1990-07, Vol.249 (4965), p.174-177

Ort / Verlag

Washington, DC: American Association for the Advancement of Science

Erscheinungsjahr

1990

Quelle

MEDLINE

Beschreibungen/Notizen

• T cell hybridomas that express zeta zeta, but not zeta eta, dimers in their T cell receptors (TCRs) produce interleukin-2 (IL-2) and undergo an inhibition of spontaneous growth when activated by antigen, antibodies to the receptor, or antibodies to Thy-1. Hybridomas without zeta and eta were reconstituted with mutated zeta chains. Cytoplasmic truncations of up to 40% of the zeta molecule reconstituted normal surface assembly of TCRs, but antigen-induced IL-2 secretion and growth inhibition were lost. In contrast, cross-linking antibodies to the TCR activated these cells. A point mutation conferred the same signaling phenotype as did the truncations and caused defective antigen-induced tyrosine kinase activation. Thus zeta allows the binding of antigen/major histocompatibility complex (MHC) to alpha beta to effect TCR signaling.