Details

Autor(en) / Beteiligte

• Wormsley, Susan B.
• Baird, Stephen M.
• Gadol, Nancy
• Rai, Kanti R.
• Sobol, Robert E.

Titel

Characteristics of CD11c+CD5+ Chronic B-Cell Leukemias and the Identification of Novel Peripheral Blood B-Cell Subsets With Chronic Lymphoid Leukemia Immunophenotypes

Ist Teil von

• Blood, 1990-07, Vol.76 (1), p.123-130

Ort / Verlag

Washington, DC: Elsevier Inc

Erscheinungsjahr

1990

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Previous studies have indicated that chronic lymphocytic leukemias (CLL) are characterized by the coexpression of CD5 and B-cell antigens, while hairy cell leukemias (HCL) typically express CD11c+CD5 B-cell immunophenotypes. In this report we describe the features of B-cell leukemias with CD11c+CD5+ immunophenotypes and the identification of novel circulating B-cell subsets defined by the expression of CD20, CDS, and CD11c antigens. Morphologic evaluation of 14 CD11c+ CD5+ B-cell leukemias showed that they generally had larger cellular diameters (14 to 21 Mm) and lower nuclearxytoplasm ratios than typical small lymphocyte CLL. These cases did not exhibit the well-defined nucleoli characteristic of prolymphocytic leukemia (PLL). The presenting clinical features of CD11c+CD5+ B-cell leukemias were most consistent with CLL or PLL, and none of the evaluated cases had pancytopenia, splenomegaly, and cytoplasmic villi characteristic of HCL. Examination of normal peripheral blood (n = 6) by three-color flow cytometry identified four novel B-cell subsets with the following immunophenotypes (mean percent of total CD20+ B cells ± SE):CD20+CD5+CD11c+ (8.0 ± 1.6); CD20+CD5-CD11c+(12.0 ± 2.0); CD20+CD5+CD11c- (35.0 ± 4.9); and CD20+CD5-CD11c- (44.0 ± 5.0). Our findings suggest that CD11c+CD5+ B-cell leukemias with atypical morphologic features represent forms of CLL or PLL rather than HCL. In addition, we have identified novel subsets of circulating B cells defined by patterns of CD20, CD5, and CD11c expression that correspond to the immunophenotypes of chronic B-cell leukemias.