Details

Autor(en) / Beteiligte

• Habus, Ivan
• Xie, Jin
• Iyer, Radhakrishnan P
• Zhou, Wen-Qiang
• Shen, Ling X
• Agrawal, Sudhir

Titel

A Mild and Efficient Solid-Support Synthesis of Novel Oligonucleotide Conjugates

Ist Teil von

• Bioconjugate chemistry, 1998-03, Vol.9 (2), p.283-291

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

1998

Quelle

MEDLINE

Beschreibungen/Notizen

• Conjugates of oligodeoxyribonucleotide phosphorothioate (ODN-PS) with folic acid, retinoic acid, arachidonic acid, and methoxypoly(ethylene glycol)propionic acid have been synthesized. The procedure involved the initial solid-phase preparation of 5‘-amino-functionalized ODN-PS using N-pent-4-enoyl-derived (PNT) nucleoside phosphoramidites followed by conjugation of the oligonucleotide either to the ligand acids, using 1-[3-(dimethylamino)propyl]-3-ethylcarbodiimide as a coupling reagent, or to their corresponding succinimidyl derivatives. Subsequent exposure of the support to aqueous ammonium hydroxide (28%, 2 h, 55 °C) resulted in the release of the fully deprotected ODN conjugates, which were purified by reversed-phase HPLC or by preparative polyacrylamide gel electrophoresis. The identity of the oligonucleotide conjugates was confirmed by MALDI-TOF mass spectral analysis.