Journal of neuroimmunology, 1998, Vol.81 (1), p.184-192
1998
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Distribution of nociceptin/orphanin FQ receptor transcript in human central nervous system and immune cells
- Ist Teil von
- Journal of neuroimmunology, 1998, Vol.81 (1), p.184-192
- Ort / Verlag
- Netherlands: Elsevier B.V
- Erscheinungsjahr
- 1998
- Quelle
- Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
- Beschreibungen/Notizen
- We have examined the distribution of the opioid receptor-like-1 (ORL-1) transcript in the human CNS as well as human immune cells by RT-PCR and RNAse protection. The hORL-1 mRNA was distributed throughout the brain and particularly abundant in cortical areas, striatum, thalamus and hypothalamus. In the immune system, gene transcription was observed in normal circulating lymphocytes and monocytes as well as in T, B and monocytic cell lines. A splice variant, lacking 15 nucleotides at the junction between exon 1 and exon 2, showed a distribution similar to the already known ORL-1 transcript. Altogether these results show comparable expression levels of the hORL-1 gene in both nervous and immune systems, suggesting that the ORL-1-encoded receptor may participate to neuronal and non-neuronal physiological functions in humans.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0165-5728eISSN: 1872-8421DOI: 10.1016/S0165-5728(97)00178-1Titel-ID: cdi_proquest_miscellaneous_79754085
- Format
- –
- Schlagworte
- Adult, Amino Acid Sequence, Base Sequence, Brain Chemistry, Cell Line, Cloning, Molecular, Distribution, Female, Human, Humans, Male, Middle Aged, Molecular Sequence Data, Nerve Tissue Proteins - analysis, Nerve Tissue Proteins - genetics, Neuroimmunomodulation, Nociceptin, Nociceptin/orphanin FQ receptor, Opioid Peptides - analysis, Opioid Peptides - genetics, Opioid receptors, Organ Specificity, Polymerase Chain Reaction, RNA Splicing, RNA, Messenger - analysis, RT-PCR, Sequence Alignment, Sequence Homology, Solution hybridization