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Prenatal hippocampal granule cells in primary cell culture form mossy fiber boutons at pyramidal cell dendrites
Journal of neuroscience research, 1998-03, Vol.51 (5), p.602-611
Grosse, Gisela
Tapp, Rosemarie
Wartenberg, Maria
Sauer, Heinrich
Fox, Petra A.
Grosse, Johannes
Gratzl, Manfred
Bergmann, Mathias
1998
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Grosse, Gisela
Tapp, Rosemarie
Wartenberg, Maria
Sauer, Heinrich
Fox, Petra A.
Grosse, Johannes
Gratzl, Manfred
Bergmann, Mathias
Titel
Prenatal hippocampal granule cells in primary cell culture form mossy fiber boutons at pyramidal cell dendrites
Ist Teil von
Journal of neuroscience research, 1998-03, Vol.51 (5), p.602-611
Ort / Verlag
New York: John Wiley & Sons, Inc
Erscheinungsjahr
1998
Quelle
MEDLINE
Beschreibungen/Notizen
Mossy fiber boutons are the sites of synaptic signalling between hippocampal granule and pyramidal neurons. We studied the formation and localization of these terminals during development of prenatal hippocampal neurons in primary culture. Using the synaptic vesicle membrane proteins synaptophysin and synaptoporin as markers we observed that both proteins were mainly localized in perikarya and processes of fetal hippocampal neurons during the first days in vitro (DIV). Following DIV 6 synaptophysin was present in small terminals. After DIV 20 in addition large terminals immunoreactive for synaptophysin and synaptoporin were found, which were identified by electron microscopy as mossy fiber boutons impinging on pyramidal neuron dendrites. Synaptic vesicles and endosomes in the mossy fiber boutons were labeled when incubated with exogenous horseradish peroxidase, indicating that they were competent for exo‐endocytosis. Taken together, our data show that hippocampal granule neurons grown in dissociated primary cultures form mossy fiber boutons containing synaptophysin and synaptoporin at pyramidal cell dendrites. Since the composition and the characteristic morphology of mossy fiber boutons formed in vitro is the same as observed in vivo we conclude that their development follows an intrinsic program. J. Neurosci. Res. 51:602–611, 1998. © 1998 Wiley‐Liss, Inc.
Sprache
Englisch
Identifikatoren
ISSN: 0360-4012
eISSN: 1097-4547
DOI: 10.1002/(SICI)1097-4547(19980301)51:5<602::AID-JNR7>3.0.CO;2-J
Titel-ID: cdi_proquest_miscellaneous_79733848
Format
–
Schlagworte
Animals
,
Cells, Cultured
,
Dendrites - chemistry
,
Dendrites - physiology
,
Fluorescent Antibody Technique
,
hippocampal cell culture
,
Membrane Proteins - analysis
,
Mice
,
Mice, Inbred Strains
,
Microscopy, Electron
,
Mossy Fibers, Hippocampal - chemistry
,
Mossy Fibers, Hippocampal - physiology
,
Mossy Fibers, Hippocampal - ultrastructure
,
mouse
,
Presynaptic Terminals - chemistry
,
Presynaptic Terminals - physiology
,
Pyramidal Cells - chemistry
,
Pyramidal Cells - physiology
,
Pyramidal Cells - ultrastructure
,
synaptogenesis
,
synaptophysin
,
Synaptophysin - analysis
,
synaptoporin
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