Details

Autor(en) / Beteiligte

• Rogakou, Emmy P.
• Pilch, Duane R.
• Orr, Ann H.
• Ivanova, Vessela S.
• Bonner, William M.

Titel

DNA Double-stranded Breaks Induce Histone H2AX Phosphorylation on Serine 139

Ist Teil von

• The Journal of biological chemistry, 1998-03, Vol.273 (10), p.5858-5868

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

1998

Quelle

MEDLINE

Beschreibungen/Notizen

• When mammalian cell cultures or mice are exposed to ionizing radiation in survivable or lethal amounts, novel mass components are found in the histone H2A region of two-dimensional gels. Collectively referred to as γ, these components are formed in vivo by several procedures that introduce double-stranded breaks into DNA. γ-Components, which appeared to be the only major novel components detected by mass or 32PO4incorporation on acetic acid-urea-Triton X-100-acetic acid-urea-cetyltrimethylammonium bromide or SDS-acetic acid-urea-cetyltrimethylammonium bromide gels after exposure of cells to ionizing radiation, are shown to be histone H2AX species that have been phosphorylated specifically at serine 139. γ-H2AX appears rapidly after exposure of cell cultures to ionizing radiation; half-maximal amounts are reached by 1 min and maximal amounts by 10 min. At the maximum, approximately 1% of the H2AX becomes γ-phosphorylated per gray of ionizing radiation, a finding that indicates that 35 DNA double-stranded breaks, the number introduced by each gray into the 6 × 109 base pairs of a mammalian G1 genome, leads to the γ-phosphorylation of H2AX distributed over 1% of the chromatin. Thus, about 0.03% of the chromatin appears to be involved per DNA double-stranded break. This value, which corresponds to about 2 × 106 base pairs of DNA per double-stranded break, indicates that large amounts of chromatin are involved with each DNA double-stranded break. Thus, γ-H2AX formation is a rapid and sensitive cellular response to the presence of DNA double-stranded breaks, a response that may provide insight into higher order chromatin structures.