Growth hormone & IGF research, 1998-04, Vol.8 (2), p.125-132
1998
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- Autor(en) / Beteiligte
- Titel
- Insulin-like growth factor: receptor and binding proteins in human retinal endothelial cell cultures of diabetic and non-diabetic origin
- Ist Teil von
- Growth hormone & IGF research, 1998-04, Vol.8 (2), p.125-132
- Ort / Verlag
- Scotland: Elsevier Ltd
- Erscheinungsjahr
- 1998
- Quelle
- Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
- Beschreibungen/Notizen
- Human retinal endothelial cell (HREC) cultures of diabetic and non-diabetic origin were examined for the production of insulin-like growth factor I (IGF-I), IGF-I receptor and IGF-binding proteins (IGFBPs) using colloidal gold quantitative immunocytochemistry and quantitative reverse transcriptase-polymerase chain reaction (QRT-PCR). The levels of immunoreactivity for IGF-I receptor and for four IGFBPs (IGFBP-1,-2,-3 and-5) were significantly increased in diabetic HREC cultures. Moreover, diabetic HREC cultures showed significantly less immunoreactivity for IGF-I and for IGFBP-4 as compared to non-diabetic HREC cultures. Message levels for IGF-I decreased two-fold in diabetic HREC and correlated with protein levels. Message levels for IGFBP-1, -2 and -5 increased 1.5-, 1.7- and 1.6-fold, respectively, in diabetic HREC and correlated with protein levels. However, the protein levels for IGF-I receptor and IGFBP-3 and -4 did not correlate with mRNA levels. There were no differences in mRNA levels for IGF-I receptor and IGFBP-3 and -4 between diabetic and non-diabetic HREC cultures, suggesting a post-transcriptional regulation of IGF-I receptor and the two IGFBPs. The net effect, however, supports enhanced IGF-I action in HREC cultures of diabetic origin which is an important cellular event in diabetic retinopathy.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1096-6374eISSN: 1532-2238DOI: 10.1016/S1096-6374(98)80102-0Titel-ID: cdi_proquest_miscellaneous_79633810
- Format
- –
- Schlagworte
- Cells, Cultured, Diabetes Mellitus - metabolism, Diabetes Mellitus - pathology, Diabetic Retinopathy - metabolism, Endothelium - cytology, Endothelium - metabolism, Endothelium - ultrastructure, Female, Gene Expression Regulation, Humans, Immunohistochemistry, In Situ Hybridization, Insulin-Like Growth Factor Binding Proteins - genetics, Insulin-Like Growth Factor Binding Proteins - metabolism, Insulin-Like Growth Factor I - genetics, Insulin-Like Growth Factor I - metabolism, Male, Microscopy, Immunoelectron, Middle Aged, Receptor, IGF Type 1 - genetics, Receptor, IGF Type 1 - metabolism, Retina - cytology, Retina - metabolism, Retina - ultrastructure, Reverse Transcriptase Polymerase Chain Reaction, RNA, Messenger - metabolism