Molecular cell, 1997-12, Vol.1 (1), p.47-57
1997
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Details
- Autor(en) / Beteiligte
- Titel
- Complementation Cloning of S2P, a Gene Encoding a Putative Metalloprotease Required for Intramembrane Cleavage of SREBPs
- Ist Teil von
- Molecular cell, 1997-12, Vol.1 (1), p.47-57
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 1997
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- We report the cloning of a gene, S2P, that encodes a putative metalloprotease required for intramembrane proteolysis of sterol-regulatory element–binding proteins (SREBPs) at Site-2. SREBPs are membrane-bound transcription factors that activate genes regulating cholesterol metabolism. The active NH 2-terminal domains of SREBPs are released from membranes by sequential cleavage at two sites: Site-1, within the lumen of the endoplasmic reticulum; and Site-2, within a transmembrane segment. The human S2P gene was cloned by complementation of mutant CHO cells that cannot cleave SREBPs at Site-2 and are cholesterol auxotrophs. S2P defines a new family of polytopic membrane proteins that contain an HE XX H sequence characteristic of zinc metalloproteases. Mutation of the putative zinc-binding residues abolishes S2P activity. S2P encodes an unusual metalloprotease that cleaves proteins within transmembrane segments.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1097-2765eISSN: 1097-4164DOI: 10.1016/S1097-2765(00)80006-4Titel-ID: cdi_proquest_miscellaneous_79608594
- Format
- –
- Schlagworte
- Animals, CCAAT-Enhancer-Binding Proteins, CHO Cells - enzymology, Cloning, Molecular, Cricetinae, DNA Mutational Analysis, DNA, Complementary, DNA-Binding Proteins - metabolism, Endopeptidases - genetics, Endopeptidases - metabolism, Gene Expression Regulation, Enzymologic, Genes, Reporter, Genetic Complementation Test, Humans, Luciferases, Membrane Proteins - genetics, Membrane Proteins - metabolism, Metalloendopeptidases - genetics, Metalloendopeptidases - metabolism, Molecular Sequence Data, Mutation - physiology, Nuclear Proteins - metabolism, Sequence Homology, Amino Acid, Sterol Regulatory Element Binding Protein 1, Transcription Factors, Transfection