Details

Autor(en) / Beteiligte

• Cherdchu, Chainarong
• Deupree, Jean D.
• Hexum, Terry D.

Titel

Binding sites for 125I-neuropeptide Y (NPY) on membranes from bovine adrenal medulla

Ist Teil von

• European journal of pharmacology, 1989-12, Vol.173 (2), p.115-119

Ort / Verlag

Amsterdam: Elsevier B.V

Erscheinungsjahr

1989

Quelle

Elsevier ScienceDirect Journals

Beschreibungen/Notizen

• Bovine adrenal medulla membranes were examined for the presence of specific 125I-neuropeptide Y ( 125I-NPY) binding sites using rapid centrifugation to measure the amount of bound ligand. Specific binding was determined from the difference between 125I-NPY bound in the presence and absence of 10 −7 M unlabeled NPY. The binding was saturable and reached equilibrium within 5 min at 0°C. Analysis of specific 125I-NPY binding using the LIGAND computer program indicated a best fit for a two site model with a K d of 0.26 nM and a B max of 12 fmol/mg protein for the high affinity site and a K d of 170 nM and a B max of 6 pmol/mg protein for the low affinity site. The rate of dissociation (k −1) was 0.071/min with a t 1 2 of 9 min. Displacement curves for avian or human pancreatic polypeptide revealed that these peptides displaced 125I-NPY from both sites with IC 50 values greater than 10 nM.