The Journal of biological chemistry, 1997-12, Vol.272 (50), p.31213-31216
1997
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Details
- Autor(en) / Beteiligte
- Titel
- A Caveolar Complex between the Cationic Amino Acid Transporter 1 and Endothelial Nitric-oxide Synthase May Explain the “Arginine Paradox”
- Ist Teil von
- The Journal of biological chemistry, 1997-12, Vol.272 (50), p.31213-31216
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 1997
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Immunohistochemistry of porcine pulmonary artery endothelial cells (PAEC) with antibodies specific for caveolin, endothelial nitric-oxide synthase (eNOS), and the arginine transporter (CAT1) demonstrates that all of these proteins co-localize in plasma membrane caveolae. When incubated with solubilized PAEC plasma membrane proteins, eNOS-specific antibody immunoprecipitates CAT1-mediated arginine transport. These results document the existence of a caveolar complex between CAT1 and eNOS in PAEC that provides a mechanism for the directed delivery of substrate arginine to eNOS. Direct transfer of extracellular arginine to membrane-bound eNOS accounts for the “arginine paradox” and explains why caveolar localization of eNOS is required for optimal nitric oxide production by endothelial cells.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0021-9258eISSN: 1083-351XDOI: 10.1074/jbc.272.50.31213Titel-ID: cdi_proquest_miscellaneous_79455800
- Format
- –
- Schlagworte
- ACTIVE TRANSPORT, Amino Acid Transport Systems, Basic, Animals, ARGININA, ARGININE, Arginine - metabolism, Arginine - physiology, BINDING PROTEINS, Biological Transport, BLOOD VESSELS, Carrier Proteins - metabolism, Caveolin 1, Caveolins, Cell Membrane - metabolism, CELL MEMBRANES, Cells, Cultured, CERDO, Endothelium, Vascular - enzymology, EPITELIO, EPITHELIUM, IMMUNOCYTOCHEMISTRY, IMMUNOLOGIE, IMMUNOLOGY, INMUNOLOGIA, INTERACTIONS, Macromolecular Substances, MEMBRANAS CELULARES, MEMBRANE CELLULAIRE, Membrane Proteins - metabolism, Nitric Oxide Synthase - metabolism, OXIDOREDUCTASES, OXIDORREDUCTASAS, OXYDOREDUCTASE, OXYGENASES, PLASMA MEMBRANES, PORCIN, PROTEINAS, PROTEINAS AGLUTINANTES, PROTEINE, PROTEINE DE LIAISON, PROTEINS, PULMONARY ARTERY, SURFACE PROTEINS, SWINE, VAISSEAU SANGUIN, VASOS SANGUINEOS