Analytical biochemistry, 1989-11, Vol.182 (2), p.200-206
1989
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Details
- Autor(en) / Beteiligte
- Titel
- Novel methods to determine the epitopes on the asparagine-linked oligosaccharides of glycoproteins
- Ist Teil von
- Analytical biochemistry, 1989-11, Vol.182 (2), p.200-206
- Ort / Verlag
- San Diego, CA: Elsevier Inc
- Erscheinungsjahr
- 1989
- Quelle
- Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
- Beschreibungen/Notizen
- Oligosaccharides obtained from glycoproteins by hydrazinolysis followed by N-acetylation were covalently coupled to an amino-bonded thin-layer plate or to a poly- l-lysine coating on the microtiter plate. Bound oligosaccharides can be directly detected by immunostaining or by enzyme-linked immunosorbent assay (ELISA) using a mouse monoclonal antibody. These methods are simple and require small amounts of oligosaccharides and antibodies. The methods were successfully applied to determine the epitope of F48-60, a monoclonal antibody which reacts with the N-linked sugar chains of nonspecific cross-reacting antigen 2, but not with those of carcinoembryonic antigen. The results revealed that the antibody recognizes the Galβ1 → 3GlcNAcβ1 → group.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0003-2697eISSN: 1096-0309DOI: 10.1016/0003-2697(89)90579-4Titel-ID: cdi_proquest_miscellaneous_79435241
- Format
- –
- Schlagworte
- Analytical biochemistry: general aspects, technics, instrumentation, Analytical, structural and metabolic biochemistry, Animals, Antibodies, Monoclonal - immunology, Asparaginase, Asparagine, Biological and medical sciences, Cattle, Chromatography, Thin Layer, Enzyme-Linked Immunosorbent Assay, Epitopes - analysis, Fundamental and applied biological sciences. Psychology, Glycoproteins - analysis, Humans, Mice, Microchemistry, Oligosaccharides - immunology, Polylysine - metabolism