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Application of Ion-Pair High Performance Liquid Chromatography for Analysis of Hyoscyamine and Scopolamine in Solanaceous Crude Drugs
Ist Teil von
Chemical and Pharmaceutical Bulletin, 1989/09/25, Vol.37(9), pp.2456-2458
Ort / Verlag
Tokyo: The Pharmaceutical Society of Japan
Erscheinungsjahr
1989
Quelle
MEDLINE
Beschreibungen/Notizen
A new ion-pair high-performance liquid chromatographic method for the analysis of hyoscyamine and scopolamine in various kinds of crude drugs derived from solanaceous plants was evaluated using sodium dodecyl sulfate as a counter ion. A reversed-phase chromatographic system consisting of a chemically bonded ODS silica gel column with phosphate buffer (pH 2.5)-acetonitrile (65 : 35) containing 17.5mM sodium dodecyl sulfate as the mobile phase was used. Hyoscyamine and scopolamine in crude drugs derived from Scopolia, Atropa, Hyoscyamus and Datura species were separated from other compounds in the crude drugs and determined within 20 min after direct injection of the solution extracted with the mobile phase. The results for various kinds of samples are presented.