Details

Autor(en) / Beteiligte

• Peale, Franklin V
• Ishibe, Yuko
• Klinge, Carolyn M
• Zain, Sayeeda
• Hilf, Russell
• Bambara, Robert A

Titel

Rapid purification of the estrogen receptor by sequence-specific DNA affinity chromatography

Ist Teil von

• Biochemistry (Easton), 1989-10, Vol.28 (22), p.8671-8675

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

1989

Quelle

MEDLINE

Beschreibungen/Notizen

• Rapid purification of calf uterine estrogen receptor (ER) to near homogeneity has been accomplished by use of sequence-specific DNA affinity resin. Very high selectivity for the estrogen receptor is achieved through the use of DNA-Sepharose containing eight tandem copies of a consensus estrogen response element (ERE) DNA sequence. The highly purified ER prepared by this new scheme may be labeled economically with ligands of high specific activity. This purification scheme selects for intact receptors retaining function in both estrogen-binding and DNA-binding domains. Purified receptor has an electrophoretic mobility consistent with a molecular weight of 68,000, sediments as a 5S species on sucrose gradients, and reacts with antibody specific to the human estrogen receptor.