Details

Autor(en) / Beteiligte

• Merćep, Mladen
• Weissman, Allan M.
• Frank, Stuart J.
• Klausner, Richard D.
• Ashwell, Jonathan D.

Titel

Activation-Driven Programmed Cell Death and T Cell Receptor $\zeta \eta $ Expression

Ist Teil von

• Science (American Association for the Advancement of Science), 1989-12, Vol.246 (4934), p.1162-1165

Ort / Verlag

United States: The American Association for the Advancement of Science

Erscheinungsjahr

1989

Quelle

MEDLINE

Beschreibungen/Notizen

• Activation of spontaneously dividing T cell hybridomas induces interleukin-2 (IL-2) production, a cell cycle block, and programmed cell death. T cell hybridomas that express the T cell antigen receptor (TCR) $\zeta $ homodimer ($\zeta _{2}$), but not the TCR $\zeta \eta $ heterodimer, were studied. The $\zeta \eta ^{-}$ cells produced little or no inositol phosphates (IP) when stimulated with antigen. In most cases the hydrolysis of phosphoinositides was also impaired after stimulation with antibody to CD3, although one $\zeta \eta ^{-}$ cell produced normal concentrations of IP. The $\zeta \eta ^{-}$ cells slowed their growth and secreted IL-2 in response to both stimuli. However, the $\zeta \eta ^{-}$ cells did not die after activation with antigen. Since activated thymocytes also undergo programmed cell death, these results may have important implications for the role of the $\zeta \eta $ TCR in negative selection.