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Dynamic and thermodynamic effects of glycerol on bovine serum albumin in aqueous solution: a tryptophan phosphorescence study
Ist Teil von
Journal of photochemistry and photobiology. B, Biology, 1997-08, Vol.40 (1), p.55-60
Ort / Verlag
Switzerland: Elsevier B.V
Erscheinungsjahr
1997
Link zum Volltext
Quelle
Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
Beschreibungen/Notizen
The phosphorescence decay of bovine serum albumin in water-glycerol solutions at room temperature is analysed by the maximum entropy method. While in pure water the decay was found to be quasi-monoexponential, in water-glycerol mixtures it is associated with more complex lifetime distributions. This is a direct proof for the existence of multiple protein conformers. It was also found that the increase of the glycerol concentration induces a continuous shift of the lifetime pattern to longer lifetimes. This effect is analysed in connection with the coupling between the solvent viscosity and the internal protein mobility.