FEBS letters, 1997-07, Vol.411 (1), p.63-66
1997
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Details
- Autor(en) / Beteiligte
- Titel
- Bcl-2 protein inhibits oxysterol-induced apoptosis through suppressing CPP32-mediated pathway
- Ist Teil von
- FEBS letters, 1997-07, Vol.411 (1), p.63-66
- Ort / Verlag
- England: Elsevier B.V
- Erscheinungsjahr
- 1997
- Quelle
- Wiley Online Library - AutoHoldings Journals
- Beschreibungen/Notizen
- Oxysterols are presumed to mediate cytotoxicity of oxidized LDL in atherosclerotic lesions. To elucidate its molecular mechanism, we established murine macrophage-like P388-D1 cells which over-express Bcl-2 protein by retrovirus-mediated gene transfer. Oxysterols (7-ketocholesterol, 25-hydroxycholesterol) induced nuclear condensation and oligonucleosomal DNA fragmentation, which were partially inhibited by Bcl-2 over-expression. Though CPP32 inhibitor suppressed the cell death in control cells, it showed no additive protection in the cells over-expressing Bcl-2. These findings indicate that oxysterols induce apoptosis via Bcl-2-inhibitable and -uninhibitable pathways, and the former depends on CPP32 activation.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0014-5793eISSN: 1873-3468DOI: 10.1016/S0014-5793(97)00662-5Titel-ID: cdi_proquest_miscellaneous_79173332
- Format
- –
- Schlagworte
- 3-hydroxy-3-methylglutalyl coenzyme A, Ac-DEVD-CHO, acetyl-Asp-Glu-Val-Asp-CHO, Animals, Apoptosis, Bcl-2, Caspase 3, Caspases, Cell Line, Cysteine Endopeptidases - metabolism, Cysteine Proteinase Inhibitors - pharmacology, Cytotoxicity, HMG-CoA, Hydroxycholesterols - antagonists & inhibitors, Hydroxycholesterols - pharmacology, ICE, interleukin-1β converting enzyme, Ketocholesterols - antagonists & inhibitors, Ketocholesterols - pharmacology, LDL, low density lipoprotein, Macrophages - cytology, Macrophages - drug effects, Mice, Oligopeptides - pharmacology, Oxysterol, Proto-Oncogene Proteins c-bcl-2 - genetics, Proto-Oncogene Proteins c-bcl-2 - metabolism