Details

Autor(en) / Beteiligte

• Block-Galarza, Jessie
• Chase, Kathryn O
• Sapp, Ellen
• Vaughn, Kevin T
• Vallee, Richard B
• DiFiglia, Marian
• Aronin, Neil

Titel

Fast transport and retrograde movement of huntingtin and HAP 1 in axons

Ist Teil von

• Neuroreport, 1997-07, Vol.8 (9), p.2247-2250

Ort / Verlag

Hagerstown, MD: Lippincott-Raven Publishers

Erscheinungsjahr

1997

Quelle

MEDLINE

Beschreibungen/Notizen

• HUNTINGTIN, the protein product of the Huntingtonʼs disease gene, associates with vesicle membranes and microtubules in neurons. Analysis of axonal transport with a stop-flow, double crush ligation approach in rat sciatic nerve showed that full length huntingtin (350 kDa) and an N-terminal cleavage product (50 kD) were increased within 6–12 h on both the proximal and distal sides of the crush site when compared with normal unligated nerve. The huntingtin associated protein HAP 1 and the retrograde motor protein dynein also accumulated on both sides of the crush, whereas the vesicle docking protein SNAP-25 was elevated only proximally. The cytoskeletal protein a-tubulin was unaffected. The rapid anterograde accumulation of huntingtin and HAP 1 is compatible with their axonal transport on vesicular membranes. Retrograde movement of both proteins, as seen by accumulation distal to the nerve crush, may be necessary for their degradation at the soma or for a function in retrograde membrane trafficking.