Details

Autor(en) / Beteiligte

• Funk, Gregory D
• Kanjhan, Refik
• Walsh, Carmen
• Lipski, Janusz
• Comer, Alison M
• Parkis, Marjorie A
• Housley, Gary D

Titel

P2 Receptor Excitation of Rodent Hypoglossal Motoneuron Activity In Vitro and In Vivo: A Molecular Physiological Analysis

Ist Teil von

• The Journal of neuroscience, 1997-08, Vol.17 (16), p.6325-6337

Ort / Verlag

United States: Soc Neuroscience

Erscheinungsjahr

1997

Quelle

MEDLINE

Beschreibungen/Notizen

• The role of P2 receptors in controlling hypoglossal motoneuron (XII MN) output was examined (1) electrophysiologically, via application of ATP to the hypoglossal nucleus of rhythmically active mouse medullary slices and anesthetized adult rats; (2) immunohistochemically, using an antiserum against the P2X2 receptor subunit; and (3) using PCR to identify expression of P2X2 receptor subunits in micropunches of tissue taken from the XII motor nucleus. Application of ATP to the hypoglossal nucleus of mouse medullary slices and anesthetized rats produced a suramin-sensitive excitation of hypoglossal nerve activity. Additional in vitro effects included potentiation of inspiratory hypoglossal nerve output via a suramin- and pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid (PPADS)-sensitive mechanism, XII MN depolarization via activation of a suramin-sensitive inward current, decreased neuronal input resistance, and a slow-onset theophylline-sensitive reduction of inspiratory output likely resulting from hydrolysis of extracellular ATP to adenosine and activation of P1 receptors. Immunohistochemically, P2X2 receptors were detected in inspiratory XII MNs that were labeled with Lucifer yellow. These data, combined with identification of mRNA for three P2X2 receptor subunit isoforms within the hypoglossal nucleus (two of which have not been localized previously in brain) and the previous demonstration that P2X receptors are ubiquitously expressed in cranial and spinal motoneuron pools, support not only a role of P2 receptors in modulating inspiratory hypoglossal activity but a general role of P2 receptors in modulating motor outflow from the CNS.