Details

Autor(en) / Beteiligte

• Beier, David R.
• Morton, Cynthia C.
• Leder, Aya
• Wallace, Racheal
• Leder, Philip

Titel

Perinatal lethality ( ple): A mutation caused by integration of a transgene into distal mouse chromosome 15

Ist Teil von

• Genomics (San Diego, Calif.), 1989-05, Vol.4 (4), p.498-504

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

1989

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• We have used cytogenetic and recombinational analysis to determine the position of a transgene integrated into the mouse genome. The transgene maps to band F on the physical map of mouse chromosome 15 by in situ analysis and is tightly linked genetically to a cluster of loci that include the mutations caracul ( Ca) and microcytic anemia ( mk). Genetic analysis of the offspring of noninbred animals carrying the transgene and marker loci demonstrates a significant deficiency of homozygous progeny at weaning. When inbred mice heterozygous for the transgene are mated, about one-quarter of their offspring are homozygous; none of these animals survives more than 1 day after birth. It appears likely that a recessive insertional mutation has occurred as a result of transgene integration into a locus required for postnatal viability. We call this mutation trans genic perinatal lethality ( Tg.ple).