Science (American Association for the Advancement of Science), 1989-06, Vol.244 (4910), p.1342-1344
1989
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Details
- Autor(en) / Beteiligte
- Titel
- Recombinant Gene Expression in vivo within Endothelial Cells of the Arterial Wall
- Ist Teil von
- Science (American Association for the Advancement of Science), 1989-06, Vol.244 (4910), p.1342-1344
- Ort / Verlag
- Washington, DC: The American Association for the Advancement of Science
- Erscheinungsjahr
- 1989
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- A technique for the transfer of endothelial cells and expression of recombinant genes in vivo could allow the introduction of proteins of therapeutic value in the management of cardiovascular diseases. Porcine endothelial cells expressing recombinant $\beta $-galactosidase from a murine amphotropic retroviral vector were introduced with a catheter into denuded iliofemoral arteries of syngeneic animals. Arterial segments explanted 2 to 4 weeks later contained endothelial cells expressing $\beta $-galactosidase, an indication that they were successfully implanted on the vessel wall.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0036-8075eISSN: 1095-9203DOI: 10.1126/science.2499928Titel-ID: cdi_proquest_miscellaneous_79053612
- Format
- –
- Schlagworte
- Analysis, Animals, Arteries, Atherosclerosis, Balloons, beta-Galactosidase - biosynthesis, Biological and medical sciences, Blood vessels, Care and treatment, Catheterization, Peripheral, Catheters, Cell lines, Circulatory system, Cultured cells, DNA, Recombinant, Endothelial cells, Endothelium, Endothelium, Vascular - cytology, Endothelium, Vascular - enzymology, Endothelium, Vascular - transplantation, Female, Fundamental and applied biological sciences. Psychology, Galactosidases - biosynthesis, Gene expression, Gene therapy, Genetic recombination, Genetic Vectors, Genetics, Health aspects, Iliac Artery - cytology, Laboratory staining techniques, Medical research, Molecular and cellular biology, Molecular genetics, pigs, Retroviridae, Swine, Swine, Miniature, Therapy, Thrombosis, Vascular endothelium