Biochemical and biophysical research communications, 1989-06, Vol.161 (2), p.633-639
1989
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Details
- Autor(en) / Beteiligte
- Titel
- Effect of phenylarsine oxide on protein synthesis in 3T3-L1 adipocytes
- Ist Teil von
- Biochemical and biophysical research communications, 1989-06, Vol.161 (2), p.633-639
- Ort / Verlag
- San Diego, CA: Elsevier Inc
- Erscheinungsjahr
- 1989
- Quelle
- Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
- Beschreibungen/Notizen
- In this report, we show that insulin stimulated the incorporation of tracer [ 3H]leucine into protein of 3T3-L1 adipocytes within 2 min of insulin addition. The concentration of insulin required to elicit 50% activation was 4nM. Phenylarsine oxide, an inhibitor of insulin-stimulated glucose transport, blocked not only insulin-stimulated protein synthesis but constitutive protein synthesis as well (Ki, 3μM). Importantly, protein synthesis was not required for insulin-activated glucose transport since cycloheximide added either before or after insulin had no effect on the stimulated rates of glucose transport.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0006-291XeISSN: 1090-2104DOI: 10.1016/0006-291X(89)92646-6Titel-ID: cdi_proquest_miscellaneous_79034731
- Format
- –
- Schlagworte
- adipocytes, Adipose Tissue - drug effects, Adipose Tissue - metabolism, Animals, Arsenicals - pharmacology, Biological and medical sciences, Biological Transport - drug effects, Cell Line, Cell metabolism, cell oxidation, Cell physiology, Cycloheximide - pharmacology, Fundamental and applied biological sciences. Psychology, Glucose - metabolism, In Vitro Techniques, insulin, Insulin Antagonists, Mice, Molecular and cellular biology, Protein Biosynthesis