Gene, 1997-04, Vol.189 (2), p.159-162
1997
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Details
- Autor(en) / Beteiligte
- Titel
- A bacterial cloning vector using a mutated Aequorea green fluorescent protein as an indicator
- Ist Teil von
- Gene, 1997-04, Vol.189 (2), p.159-162
- Ort / Verlag
- Netherlands: Elsevier B.V
- Erscheinungsjahr
- 1997
- Quelle
- Access via ScienceDirect (Elsevier)
- Beschreibungen/Notizen
- The bacterial cloning vector, pGreenscript A, derived from the mutated Aequorea green fluorescent protein (GFP-S65A) gene, when expressed in E. coli produced colonies that showed yellow color under daylight and strong green fluorescence under long-wave ultraviolet light. The vector was used to select for inserted foreign genes based on the loss of the yellow color/green fluorescence of E. coli cells caused by the insertional inactivation of GFP production.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0378-1119eISSN: 1879-0038DOI: 10.1016/S0378-1119(96)00753-6Titel-ID: cdi_proquest_miscellaneous_79031031
- Format
- –
- Schlagworte
- Aequorea, Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, Escherichia coli, Escherichia coli - genetics, Gene expression, Genetic Vectors - genetics, Green Fluorescent Proteins, Indicators and Reagents, Luminescent Proteins - genetics, Luminescent Proteins - metabolism, Molecular Sequence Data, pBluescript, Recombinant DNA, Reporter gene, Scyphozoa - genetics, Temperature dependence