Details

Autor(en) / Beteiligte

• Becker, Kevin G
• Nagle, James W
• Canning, Rachel D
• Dehejia, Anindya M
• Polymeropoulos, Mihael H
• Gado, Ameer M
• Biddison, William E
• Drew, Paul D

Titel

Molecular Cloning and Mapping of a Novel Human KRAB Domain-Containing C2H2-Type Zinc Finger to Chromosome 7q36.1

Ist Teil von

• Genomics (San Diego, Calif.), 1997-05, Vol.41 (3), p.502-504

Ort / Verlag

San Diego, CA: Elsevier Inc

Erscheinungsjahr

1997

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• C2H2 zinc finger proteins constitute the largest family of transcription factors known with an estimated 300-500 genes encoding these proteins in the human genome. These proteins possess two conserved cysteines that are part of an antiparallel beta-sheet and two conserved histidines that are part of an alpha-helix, coordinated by a central zinc atom to form a globular domain. The protein family is typified by the RNA polymerase II transcription factor TFIIIA and the gap gene product Kruppel. C2H2 zinc finger proteins are believed to affect gene expression through sequence-specific binding to DNA and/or RNA and through protein-protein interactions. Individual proteins have been shown to be important in development, tumorigenesis, RNA metabolism, and chromatin assembly. However, the function of most C2H2-type zinc finger proteins, and the genes they regulate, is unknown. In addition to the conserved zinc finger domain, some C2H2-type zinc finger proteins also contain conserved amino acid domains including a TGEKP element present between adjacent fingers, as well as KRAB, tramtrack, POZ, and homeodomains, found outside of the zinc finger domain. Previously, we isolated a large number of novel cDNAs that encode C2H2-type zinc finger proteins. Currently, we are involved in mapping and otherwise characterizing these cDNAs. Here, we describe the mapping and molecular characterization of a novel clone, C2H2-150, isolated from a human brain cDNA library. C2H2-150 possesses a KRAB domain and maps to chromosome 7q36.1.