Details

Autor(en) / Beteiligte

• Clerici, Mario
• Stocks, Naomi I
• Zajac, Robert A
• Boswell, R. Neal
• Bernstein, Denise C
• Mann, Dean L
• Shearer, Gene M
• Berzofsky, Jay A

Titel

Interleukin-2 production used to detect antigenic peptide recognition by T-helper lymphocytes from asymptomatic HIV-seropositive individuals

Ist Teil von

• Nature (London), 1989-06, Vol.339 (6223), p.383-385

Ort / Verlag

London: Nature Publishing

Erscheinungsjahr

1989

Quelle

MEDLINE

Beschreibungen/Notizen

• T lymphocytes from mice and healthy humans immunized against the human immunodeficiency virus (HIV) envelope have recently been shown to recognize two antigenic regions of the gp160 HIV-envelope protein which have been located on the basis of amphipathicity. In HIV-infected humans, T-cell proliferative responses are lost soon after infection. Here we demonstrate that interleukin-2 production is often retained even when proliferative activity is absent, and that it can be used to monitor T-helper cell responses by HIV-seropositive donors. We use this approach to investigate the T-helper cell response of 42 asymptomatic HIV-seropositive patients to four synthetic gp160 peptides and to influenza A virus, an antigen requiring intact CD4 T-helper cell function. As many as 67% of the HIV-seropositive donors who retain responsiveness to influenza A virus respond to a single peptide, and 85-90% responded to at least one of the peptides.