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The distribution of interspersed repetitive DNA sequences in the human genome
Ist Teil von
Genomics (San Diego, Calif.), 1989-04, Vol.4 (3), p.273-289
Ort / Verlag
United States: Elsevier Inc
Erscheinungsjahr
1989
Quelle
Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
Beschreibungen/Notizen
The distribution of interspersed repetitive DNA sequences in the human genome has been investigated, using a combination of biochemical, cytological, computational, and recombinant DNA approaches. “Low-resolution” biochemical experiments indicate that the general distribution of repetitive sequences in human DNA can be adequately described by models that assume a random spacing, with an average distance of 3 kb. A detailed “high-resolution” map of the repetitive sequence organization along 400 kb of cloned human DNA, including 150 kb of DNA fragments isolated for this study, is consistent with this general distribution pattern. However, a higher frequency of spacing distances greater than 9.5 kb was observed in this genomic DNA sample. While the overall repetitive sequence distribution is best described by models that assume a random distribution, an analysis of the distribution of
Alu repetitive sequences appearing in the GenBank sequence database indicates that there are local domains with varying
Alu placement densities.
In situ hybridization to human metaphase chromosomes indicates that local density domains for
Alu placement can be observed cytologically. Centric heterochromatin regions, in particular, are at least 50-fold underrepresented in
Alu sequences. The observed distribution for repetitive sequences in human DNA is the expected result for sequences that transpose throughout the genome, with local regions of “preference” or “exclusion” for integration.