Journal of Chromatography A, 1997-02, Vol.763 (1), p.187-192
1997
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Quantification of urinary uric acid in the presence of thymol and thimerosal by high-performance liquid chromatography
- Ist Teil von
- Journal of Chromatography A, 1997-02, Vol.763 (1), p.187-192
- Ort / Verlag
- Amsterdam: Elsevier B.V
- Erscheinungsjahr
- 1997
- Quelle
- Access via ScienceDirect (Elsevier)
- Beschreibungen/Notizen
- A high-performance liquid chromatographic method was developed as an alternative to automated enzymatic analysis of uric acid in human urine preserved with thymol and/or thimerosal. Uric acid (t R = 10min) and creatinine (t R = 5min) were separated and quantified during isocratic elution (0.025M acetate buffer, pH 4.5) from a μBondapak C 18 column. The uric-acid peak was identified chemically by incubating urine samples with uricase. The thymol/thimerosal peak appeared at 31 min during the washing step and did not interfere with the analysis. We validated the high-performance liquid chromatographic method for linearity, precision and accuracy, and the results were found to be excellent.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0021-9673DOI: 10.1016/S0021-9673(96)00740-6Titel-ID: cdi_proquest_miscellaneous_78943004
- Format
- –
- Schlagworte
- Analytical chemistry, Biological and medical sciences, Chemistry, Chromatographic methods and physical methods associated with chromatography, Chromatography, High Pressure Liquid - methods, Creatinine, Creatinine - urine, Drug Stability, Exact sciences and technology, Humans, Hydrogen-Ion Concentration, Medical sciences, Nephrology. Urinary tract diseases, Other chromatographic methods, Preservatives, Pharmaceutical, Sensitivity and Specificity, Space life sciences, Thimerosal, Thymol, Uric acid, Uric Acid - urine, Urinary system involvement in other diseases. Miscellaneous, Urinary tract. Prostate gland