Current microbiology, 1997-05, Vol.34 (5), p.280-283
1997
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Details
- Autor(en) / Beteiligte
- Titel
- 31P NMR identification of metabolites and pH determination in the cyanobacterium Synechocystis sp. PCC 6308
- Ist Teil von
- Current microbiology, 1997-05, Vol.34 (5), p.280-283
- Ort / Verlag
- New York, NY: Springer
- Erscheinungsjahr
- 1997
- Quelle
- SpringerLINK Contemporary (Konsortium Baden-Württemberg)
- Beschreibungen/Notizen
- The identity of a number of phosphorus-containing metabolites present in Synechocystis sp. PCC 6308 has been confirmed by 31P NMR spectroscopy. The presence of D-ribulose 1,5-bisphosphate (RuBP); DL-glyceraldehyde 3-phosphate (GlyP); D(-)3-phosphoglyceric acid (3PGA); D-ribulose 5-phosphate (Ru5P);6-phosphogluconic acid (6PGA); phosphoenolpyruvate (PEP); inorganic phosphate (Pi); uridine diphosphoglucose (UDPG); ADP and ATP were demonstrated by the pH dependence of their 31P NMR chemical shifts in spectra of perchloric acid cell extracts. Intracellular pH of cells was determined to be 7.5-7.7.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0343-8651eISSN: 1432-0991DOI: 10.1007/s002849900182Titel-ID: cdi_proquest_miscellaneous_78942417
- Format
- –
- Schlagworte
- Adenosine Diphosphate - metabolism, Adenosine Triphosphate - metabolism, Bacteriology, Biological and medical sciences, Biotechnology, Cyanobacteria - metabolism, Fundamental and applied biological sciences. Psychology, Gluconates - metabolism, Glyceraldehyde 3-Phosphate - metabolism, Glyceric Acids - metabolism, Hydrogen-Ion Concentration, Intracellular Fluid - metabolism, Magnetic Resonance Spectroscopy, Metabolism. Enzymes, Metabolites, Microbiology, NMR, Nuclear magnetic resonance, Phosphates - metabolism, Phosphoenolpyruvate - metabolism, Phosphorus - metabolism, Ribulosephosphates - metabolism, Spectrum analysis, Uridine Diphosphate Glucose - metabolism