Details

Autor(en) / Beteiligte

• Hill, S A
• Stahl, M M
• Stahl, F W

Titel

Single-strand DNA intermediates in phage lambda's Red recombination pathway

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 1997-04, Vol.94 (7), p.2951-2956

Ort / Verlag

United States: National Academy of Sciences

Erscheinungsjahr

1997

Quelle

MEDLINE

Beschreibungen/Notizen

• An assay was developed to assess early intermediates arising in lambda's Red recombination pathway. Double-strand breaks were delivered in vivo to nonreplicating lambda chromosomes. Analysis by blot hybridization of total DNA extracts revealed the following: (i) long (>1.4 kilobases) single-strand DNA (ssDNA) intermediates; (ii) resection proceeding bidirectionally from the break site; (iii) single-strand overhangs of 3' polarity; and (iv) in the absence of lambda's ninR functions, a requirement of the red alpha gene product for the production of ssDNA. Therefore, the physical characteristics exhibited by these ssDNA molecules are consistent with their being an early recombination intermediate in the Red recombination pathway as proposed previously from genetic and in vitro biochemical analyses.