Details

Autor(en) / Beteiligte

• Temple, RosemaryC
• Luzio, StephenD
• Schneider, AnneroseE
• Carrington, ChristineA
• Owens, DavidR
• Sobey, WendyJ
• Nicholas Hales, C

Titel

INSULIN DEFICIENCY IN NON-INSULIN-DEPENDENT DIABETES

Ist Teil von

• The Lancet (British edition), 1989-02, Vol.333 (8633), p.293-295

Ort / Verlag

London: Elsevier Ltd

Erscheinungsjahr

1989

Quelle

MEDLINE

Beschreibungen/Notizen

• A highly specific two-site immunoradiometric assay for insulin was used to measure the plasma insulin response to 75 g glucose administered orally to 49 patients with non-insulin-dependent diabetes (NIDDM). The plasma insulin concentration 30 min after glucose ingestion was lower in the diabetic patients than in matched controls for both non-obese (11-83 pmol/l vs 136-297 pmol/l, p <0·01) and obese subjects (23-119 pmol/l vs 137-378 pmol/l, p <0·01). By means of another two-site immunoradiometric assay, the basal intact proinsulin level was found to be higher in the NIDDM patients than in the controls for both non-obese (7·1 [SEM 1·2] pmol/l vs 2·4 [0·4] pmol/l, p<0·01) and obese subjects (14·4 [2·2] pmol/l vs 5·9 [1·9] pmol/l, p<0·01). The basal level of 32-33 split proinsulin was also raised in NIDDM. Previous failure to show clear separation between normal and NIDDM insulin responses was probably due to the high concentrations of proinsulin-like molecules in the plasma of NIDDM patients. These substances cross-react as insulin in most, if not all, insulin radioimmunoassays but have very little biological insulin-like activity. It is therefore now possible and necessary to designate most NIDDM patients as insulin deficient.