Details

Autor(en) / Beteiligte

• Chen, Yao-Tseng
• Scanlan, Matthew J.
• Sahin, Ugur
• Türeci, Özlem
• Gure, Ali O.
• Tsang, Solam
• Williamson, Barbara
• Stocker, Elisabeth
• Pfreundschuh, Michael
• Old, Lloyd J.

Titel

A Testicular Antigen Aberrantly Expressed in Human Cancers Detected by Autologous Antibody Screening

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 1997-03, Vol.94 (5), p.1914-1918

Ort / Verlag

United States: National Academy of Sciences of the United States of America

Erscheinungsjahr

1997

Quelle

MEDLINE

Beschreibungen/Notizen

• Serological analysis of recombinant cDNA expression libraries (SEREX) using tumor mRNA and autologous patient serum provides a powerful approach to identify immunogenic tumor antigens. We have applied this methodology to a case of esophageal squamous cell carcinoma and identified several candidate tumor targets. One of these, NY-ESO-1, showed restricted mRNA expression in normal tissues, with high-level mRNA expression found only in testis and ovary tissues. Reverse transcription-PCR analysis showed NY-ESO-1 mRNA expression in a variable proportion of a wide array of human cancers, including melanoma, breast cancer, bladder cancer, prostate cancer, and hepatocellular carcinoma. NY-ESO-1 encodes a putative protein of Mr 17,995 having no homology with any known protein. The pattern of NY-ESO-1 expression indicates that it belongs to an expanding family of immunogenic testicular antigens that are aberrantly expressed in human cancers in a lineage-nonspecific fashion. These antigens, initially detected by either cytotoxic T cells (MAGE, BAGE, GAGE-1) or antibodies [HOM-MEL-40(SSX2), NY-ESO-1], represent a pool of antigenic targets for cancer vaccination.