Details

Autor(en) / Beteiligte

• Yu, Sui
• Mangelsdorf, Marie
• Hewett, Duncan
• Hobson, Lynne
• Baker, Elizabeth
• Eyre, Helen J
• Lapsys, Naras
• Le Paslier, Denis
• Doggett, Norman A
• Sutherland, Grant R
• Richards, Robert I

Titel

Human Chromosomal Fragile Site FRA16B Is an Amplified AT-Rich Minisatellite Repeat

Ist Teil von

• Cell, 1997-02, Vol.88 (3), p.367-374

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

1997

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Fragile sites are nonstaining gaps in chromosomes induced by specific tissue culture conditions. They vary both in population frequency and in the culture conditions required for induction. Folate-sensitive fragile sites are due to expansion of p(CCG) n trinucleotide repeats; however, the relationship between sequence composition and the chemistry of induction of fragile sites is unclear. To clarify this relationship, the distamycin A–sensitive fragile site FRA16B was isolated by positional cloning and found to be an expanded 33 bp AT-rich minisatellite repeat, p(ATATA TTATATATTATATCTAATAATATAT C/ ATA) n (consistent with DNA sequence binding preferences of chemicals that induce its cytogenetic expression). Therefore the mutation mechanism associated with trinucleotide repeats is also a property of minisatellite repeats (variable number tandem repeats).