Details

Autor(en) / Beteiligte

• MAEHARA, Reiko
• HINODE, Daisuke
• TERAI, Hiroshi
• SATO, Makoto
• NAKAMURA, Ryo
• MATSUDA, Naoki
• TANAKA, Toshiyuki
• SUGIHARA, Kunio

Titel

Inhibition of Bacterial and Mammalian Collagenolytic Activities by Tetracyclines

Ist Teil von

• Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 1988/03/28, Vol.30(1), pp.182-190

Ort / Verlag

Japan: JAPANESE SOCIETY OF PERIODONTOLOGY

Erscheinungsjahr

1988

Quelle

MEDLINE

Beschreibungen/Notizen

• Inhibitory effects of minocycline-HCl and tetracycline-HCl on collagenolytic activities of Bacteroides gingivalis, human polymorphonuclear leucocytes (PMN) and human gingival fibroblasts (HGF) were compared. Collagenolytic activities in the samples were determined using 14C-acetylated calf-tendon collagen as substrate. The enzymes were isolated from culture supernatant of B. gingivalis, PMN homogenate and culture media of HGF. At the concentration of 50μg/ml in the reaction mixture, collagenolytic activity of B. gingivalis was inhibited more than 50% of the control level by both minocycline-HCl and tetracycline-HCl. Minocycline-HCl at the concentration of 100μg/ml inhibited more strongly (65%) the collagenolytic activity of PMN than tetracycline-HCl (12%) at the same concentration. On the collagenolytic activity of HGF, inhibitory effect by both antibiotics was not observed up to the concentration of 250μg/ml. Minocycline-HCl inhibited B. gingivalis collagenolytic activity, independently of calcium ion concentration tested (5-50 mM). At the physiological calcium ion concentration (2mM), PMN collagenolytic activity was more sensitive to minocycline-HCl (70% inhibition at 50μg/ml) than HGF enzyme (20% inhibition at 500μg/ml). These results suggest that minocycline-HCl could specifically inhibit collagenolytic enzyme which may be induced during inflammatory process, without disturbing normal collagen turn over and regeneration of gingival tissue.