Details

Autor(en) / Beteiligte

• Campos-Olivas, Ramón
• Bruix, Marta
• Santoro, Jorge
• Martínez del Pozo, Alvaro
• Lacadena, Javier
• Gavilanes, JoséG.
• Rico, Manuel

Titel

Structural basis for the catalytic mechanism and substrate specificity of the ribonuclease α-sarcin

Ist Teil von

• FEBS letters, 1996-12, Vol.399 (1), p.163-165

Ort / Verlag

England: Elsevier B.V

Erscheinungsjahr

1996

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• α-Sarcin is a ribosome-inactivating protein which selectively cleaves a single phosphodiester bond in a universally conserved sequence of the major rRNA. The solution structure of α-sarcin has been determined on the basis of 1898 distance and angular experimental constraints from NMR spectroscopy. It reveals a catalytic mechanism analogous to that of the T 1 family of ribonucleases while its exquisite specificity resides in the contacts provided by its distinctive loops.