The Journal of Protozoology, 1988-11, Vol.35 (4), p.583-589
1988
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Improved method for high-yield excystation and purification of infective sporozoites of Eimeria spp
- Ist Teil von
- The Journal of Protozoology, 1988-11, Vol.35 (4), p.583-589
- Ort / Verlag
- Oxford, UK: Blackwell Publishing Ltd
- Erscheinungsjahr
- 1988
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- This report describes a new, gentle procedure for rapid and efficient excystation of large numbers of infective sporozoites of Eimeria vermiformis and Eimeria stiedai. Excysted sporozoites are purified using modifications of a previously described ion-exchange chromatography method. The procedure avoids physical breakage of oocysts and results in greater than 70% recovery of the sporozoites present as sporulated oocysts (i.e. 5-6 sporozoites per sporulated oocyst). The recovered sporozoites are greater than 95% pure and are infective in vivo. We routinely isolate greater than 2 x 10(8) sporozoites without the use of specialized or expensive equipment.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0022-3921eISSN: 2375-0804, 1550-7408DOI: 10.1111/j.1550-7408.1988.tb04156.xTitel-ID: cdi_proquest_miscellaneous_78572812
- Format
- –
- Schlagworte
- Animals, Biological and medical sciences, Chromatography, Ion Exchange - methods, EIMERIA, Eimeria - growth & development, Eimeria - isolation & purification, Eimeria - pathogenicity, EIMERIA STIEDAI, EIMERIA VERMIFORMIS, EPURATION, ESPORAS, Fundamental and applied biological sciences. Psychology, General aspects and techniques, Kinetics, MEDECINE VETERINAIRE, MEDICINA VETERINARIA, MICE, Microbiological Techniques, PARASITOLOGIA, PARASITOLOGIE, PARASITOLOGY, PATHOLOGIE, PATHOLOGY, PATOLOGIA, Protozoa, PURIFICACION, PURIFICATION, RATON, SOURIS, SPORE, SPORES, Temperature, VETERINARY MEDICINE