Details

Autor(en) / Beteiligte

• Vandenburgh, H
• Del Tatto, M
• Shansky, J
• Lemaire, J
• Chang, A
• Payumo, F
• Lee, P
• Goodyear, A
• Raven, L

Titel

Tissue-engineered skeletal muscle organoids for reversible gene therapy

Ist Teil von

• Human gene therapy, 1996-11, Vol.7 (17), p.2195-2200

Ort / Verlag

United States

Erscheinungsjahr

1996

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Genetically modified murine skeletal myoblasts were tissue engineered in vitro into organ-like structures (organoids) containing only postmitotic myofibers secreting pharmacological levels of recombinant human growth hormone (rhGH). Subcutaneous organoid implantation under tension led to the rapid and stable appearance of physiological sera levels of rhGH for up to 12 weeks, whereas surgical removal led to its rapid disappearance. Reversible delivery of bioactive compounds from postmitotic cells in tissue engineered organs has several advantages over other forms of muscle gene therapy.