Gene, 1996-10, Vol.178 (1), p.125-130
1996
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Details
- Autor(en) / Beteiligte
- Titel
- Construction by homologous recombination and phenotypic characterization of a DNA polymerase domain polA mutant of Mycobacterium smegmatis
- Ist Teil von
- Gene, 1996-10, Vol.178 (1), p.125-130
- Ort / Verlag
- Netherlands: Elsevier B.V
- Erscheinungsjahr
- 1996
- Quelle
- Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
- Beschreibungen/Notizen
- Gene replacement was achieved by homologous recombination in Mycobacterium smegmatis (Ms) using a cloned segment of the polA gene (encoding the DNA polymerase I) disrupted within the region encoding the C-terminal DNA polymerase domain by a kanamycin-resistance marker. The Ms polA755:aph mutant thus generated displayed a phenotype of hypersensitivity to DNA damage induced by UV irradiation and by hydrogen peroxide challenge.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0378-1119eISSN: 1879-0038DOI: 10.1016/0378-1119(96)00350-2Titel-ID: cdi_proquest_miscellaneous_78543036
- Format
- –
- Schlagworte
- Cloning, Molecular, DNA Damage, DNA polymerase I, DNA Polymerase I - genetics, DNA Polymerase I - metabolism, DNA repair, Gene Targeting, Homologous recombination, Hydrogen Peroxide - pharmacology, Molecular Sequence Data, Mutation, Mycobacterium - drug effects, Mycobacterium - enzymology, Mycobacterium - genetics, Mycobacterium - radiation effects, Mycobacterium smegmatis, Phenotype, polA, Recombination, Genetic, Restriction Mapping