Gene, 1996-10, Vol.176 (1), p.273-274
1996
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Details
- Autor(en) / Beteiligte
- Titel
- The nucleotide sequence of the bovine interleukin-5-encoding cDNA
- Ist Teil von
- Gene, 1996-10, Vol.176 (1), p.273-274
- Ort / Verlag
- Netherlands: Elsevier B.V
- Erscheinungsjahr
- 1996
- Quelle
- Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
- Beschreibungen/Notizen
- The nucleotide (nt) sequence encoding the bovine homologue of interleukin-5 (boIL-5) was determined. Total cDNA generated from stimulated bovine peripheral blood lymphocytes was used to amplify the boIL-5 gene using primers based on the 5' and 3' untranslated regions of the ovine IL-5 cDNA sequence. The boIL-5 coding sequence is 405 by long, coding for a 15.2 kDa precursor protein of 134 amino acids (aa). Cleavage of a putative signal peptide of 19 aa yields a mature form of 13.1 kDa. Comparisons at the nt level revealed 96%, 81%, 74% and 73% identity with the ovine, human, mouse and rat IL-5 sequences, respectively, and 97%, 66%, 59% and 58% aa identity, respectively.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0378-1119eISSN: 1879-0038DOI: 10.1016/0378-1119(96)00252-1Titel-ID: cdi_proquest_miscellaneous_78536377
- Format
- –
- Schlagworte
- ADN, Amino Acid Sequence, Animals, Base Sequence, BOVIN, CATTLE, CODE GENETIQUE, CODIGO GENETICO, Cytokine, DNA, DNA, Complementary, Eosinophil, GANADO BOVINO, GENETIC CODE, Humans, Interleukin-5 - genetics, INTERLEUKINE, INTERLEUKINS, INTERLEUQUINAS, Molecular Sequence Data, NUCLEOTIDE SEQUENCE, Polymerase chain reaction, SECUENCIA NUCLEOTIDICA, Sequence Analysis, DNA, SEQUENCE NUCLEOTIDIQUE