Details

Autor(en) / Beteiligte

• Merćep, M
• Bonifacino, J S
• Garcia-Morales, P
• Samelson, L E
• Klausner, R D
• Ashwell, J D

Titel

T cell CD3-zeta eta heterodimer expression and coupling to phosphoinositide hydrolysis

Ist Teil von

• Science (American Association for the Advancement of Science), 1988-10, Vol.242 (4878), p.571-574

Ort / Verlag

United States: American Association for the Advancement of Science

Erscheinungsjahr

1988

Quelle

MEDLINE

Beschreibungen/Notizen

• The T cell antigen receptor consists of an antigen-binding heterodimer that is noncovalently associated with at least five CD3 subunits (gamma, delta, epsilon, zeta, and eta). The CD3-zeta chains are either disulfide-linked homodimers (CD3-zeta 2) or disulfide-linked heterodimers with eta (CD3-zeta eta). Variants of a murine antigen-specific T cell hybridoma that express normal amounts of CD3-zeta 2 but decreased amounts of CD3-zeta eta were isolated. When activated, the parental cell line increased both phosphatidylinositol hydrolysis and serine-specific protein kinase activity to a much greater extent than the variants. In contrast, the activation of a tyrosine-specific kinase after stimulation with a cross-linking antibody to CD3 was similar among these cells. There was a positive linear relation between the expression of CD3-zeta eta and phosphoinositide hydrolysis stimulated by the TCR, suggesting a differential coupling of the T cell alpha beta heterodimer to signal transduction mechanisms due to alpha beta association with either CD3-zeta 2 or CD3-zeta eta.